Design and Engineering of a Multi-target (multiplex) Dna Simulant to Evaluate Nulceic Acid Based Assays for Detection of Biological Threat Agents
نویسندگان
چکیده
We designed and engineered a non-infectious Biothreat simulant that included the nucleic acid signature of Bacillus anthracis, Yersinia pestis, Coxiellla Burneti, Brucella sp., Francicella tularensis, Entherohemorragic E. coli, O157:H7, Burkholderia mallei, Burkholderia pseudomallei and Variola virus (smallpox virus). A chimera of 2040 bp was engineered to produce PCR amplicons of different sizes in a single Multiplex reaction designed for the rapid identification of the threat agents selected above.
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